The process of DNA analysis is widely employed by forensic experts for the purpose of identification and DNA
fingerprinting. DNA testing and genetic analysis play a critical role when confirming a person’s identity. Genetic evidence has to be collected before the process of DNA testing can begin.
Such evidence can be collected from one’s saliva, blood, hair, or semen samples. Once the DNA is extracted from
the samples it is then isolated from the individual cells and be analyzed utilizing various methods such as PCR and RFLP. The results of the analysis can thereafter be used for the initial and intended purpose of the DNA test.
RFLP analysis:
The restriction fragment length polymorphism (RFLP) method of DNA profiling is common with molecular
biologists. It follows a DNA sequence as it passes on to other cells. RFLP can be used in different settings such as criminal cases, helping to determine the DNA sample source.
When performing RFLP analysis, DNA samples are broken up using restriction enzymes. After this process is
complete, the restriction enzymes help separate DNA according to their lengths using gel electrophoresis. Every length of the fragment is useful for genetic analysis.
RFLP analysis has been commonly used for analyzing genetic diseases and for genome mapping analysis. RFLP
could be used to reveal a likely carrier of a disease gene or to reveal people who are at risk of certain diseases. RFLP can also be used for genetic fingerprinting.
One disadvantage of the RFLP analysis method is that it is a cumbersome and slow process that requires
enormous DNA samples to work with. It has now been replaced by more modern and cheaper DNA technology techniques.
PCR analysis:
Polymerase chain reaction (rt PCR test) is a molecular biology method employed by researchers to produce
many precise copies of a DNA sequence within a few hours from a single DNA sample. A researcher by the name of Kary Mullis developed PCR in the 1980s and this research work earned him a Nobel prize.
PCR removes the necessity of amplifying DNA using bacteria. The method is currently used in research
laboratories and medical institutions for fingerprinting, hereditary diseases diagnosis, DNA cloning, detecting and diagnosing infectious diseases, genetic engineering, and sequencing.
PCR amplifies specific DNA regions of DNA strands and involves repeated temperature cycles. In the past few years, human DNA research has developed real-time
Rt PCR test Bangalore technologies that allow for precise and automated measurements. The main method for
DNA analysis used for the vast bulk of DNA testing is Rt pcr test bangalore paternity testing, infidelity testing, ancestry testing, and relationship DNA tests are all carried out using this method.
The fact is, that the process of DNA analysis has proved indispensable in modern living and most of these
processes have become part and parcel of life itself. As the increase of crime takes over, we can be comforted of
the fact that with the advent of DNA analysis, a suspect can be brought to justice. Research on the process of
DNA analysis and testing are improving every day and better technologies are developed in order to deal with challenging situations concerning DNA profiling.